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ISO 13794:2019 Ambient air - Determination of asbestos fibres - Indirect-transfer transmission electron microscopy method >
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ISO 13794:2019 Ambient air - Determination of asbestos fibres - Indirect-transfer transmission electron microscopy method, 2019
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Symbols and abbreviated terms
- 5 Type of sample
- 6 Range
- 7 Limit of detection
- 8 Principle
- 9 Reagents
- 10 Apparatus
- 10.1 Air sampling
- 10.1.1 Filter cassette
- 10.1.2 Sampling pump
- 10.1.3 Stand
- 10.1.4 Personal sampling
- 10.1.5 Flowmeter
- 10.2 Specimen preparation laboratory
- 10.3 Equipment for analysis
- 10.3.1 Transmission electron microscope
- 10.3.2 Energy dispersive X-ray analyser
- 10.3.3 Plasma asher
- 10.3.4 Vacuum coating unit
- 10.3.5 Sputter coater
- 10.3.6 Beakers
- 10.3.7 Vacuum source
- 10.3.8 Glass filtration apparatus
- 10.3.9 Solvent washer (Jaffe washer)
- 10.3.10 Condensation washer
- 10.3.11 Slide warmer or oven
- 10.3.12 Ultrasonic bath
- 10.3.13 Carbon grating replica
- 10.3.14 Calibration specimen grids for EDXA
- 10.3.15 Carbon rod sharpener
- 10.3.16 Disposable tip micropipettes
- 10.3.17 Thermometer
- 10.3.18 Stopwatch
- 10.4 Consumable supplies
- 10.4.1 Copper or nickel electron microscope grids
- 10.4.2 Gold or nickel electron microscope grids
- 10.4.3 Aluminium foil
- 10.4.4 Carbon rod electrodes
- 10.4.5 Routine electron microscopy tools and supplies.
- 10.4.6 Reference asbestos samples
- 10.4.7 Reference samples of mineral fibres other than asbestos
- 11 Air sample collection
- 11.1 Calculation of analytical sensitivity
- 11.2 Sample collection procedure
- 12 Procedure for analysis
- 12.1 General
- 12.2 Cleaning of sample cassettes
- 12.3 Preparation of analytical filters
- 12.3.1 Selection of filter area for ashing
- 12.3.2 Ashing of sample collection filters
- 12.3.3 Aqueous dispersal of residual ash from sample collection filters
- 12.3.4 Assembly of system for filtration of aqueous dispersions
- 12.3.5 Filtration of aqueous dispersions
- 12.4 Preparation of TEM specimens from PC analytical filters
- 12.4.1 Selection of filter area for carbon-coating
- 12.4.2 Carbon-coating of filter portions
- 12.4.3 Preparation of the Jaffe washer
- 12.4.4 Placing of specimens into the Jaffe washer
- 12.5 Preparation of TEM specimens from cellulose ester analytical filters
- 12.5.1 Selection of area of filter for preparation
- 12.5.2 Preparation of solution for collapsing cellulose ester filters
- 12.5.3 Filter-collapsing procedure
- 12.5.4 Plasma etching of the filter surface
- 12.5.5 Carbon-coating
- 12.5.6 Preparation of the Jaffe washer
- 12.5.7 Placing of specimens in the Jaffe washer
- 12.6 Criteria for acceptable TEM specimen grids
- 12.7 Procedure for structure counting by TEM
- 12.7.1 General
- 12.7.2 Measurement of mean grid opening area
- 12.7.3 TEM alignment and calibration procedures
- 12.7.4 Determination of criterion for termination of TEM examination
- 12.7.5 General procedure for structure counting and size analysis
- 12.7.6 Estimation of mass concentration of asbestos fibres and bundles
- 12.7.7 Magnification requirements
- 12.8 Blank and quality control determinations
- 12.9 Calculation of results
- 13 Performance characteristics
- 13.1 General
- 13.2 Interferences and limitations of fibre identification
- 13.3 Precision and accuracy
- 13.3.1 Precision
- 13.3.2 Accuracy
- 13.3.3 Inter- and intra-laboratory analyses
- 13.4 Limit of detection
- 14 Test report
- Annex A (normative) Determination of operating conditions for plasma asher
- Annex B (normative) Determination and standardization of operating conditions for ultrasonic bath
- Annex C (normative) Calibration procedures
- Annex D (normative) Structure counting criteria
- Annex E (normative) Fibre identification procedure
- Annex F (normative) Determination of the concentration of asbestos fibres and bundles longer than 5 µm and PCM equivalent asbestos fibres
- Annex G (normative) Calculation of results
- Annex H (normative) Test procedure to determine suitability of cellulose ester sample collection filters
- Annex I (informative) Strategies for collection of air samples
- Bibliography [Go to Page]