Search book title
Filters:
BOOKS
All Books
PACKAGES
All Packages
EDITION
to
PUBLISHER
AABC
(1)
AAMI
(366)
ACI
(651)
AECDAILY
(599)
AISC
(57)
ASA
(290)
ASCE
(1055)
ASHRAE
(738)
ASME
(2283)
ASSE
(117)
ASTM
(99009)
AWC
(58)
AWWA
(650)
BHMA
(137)
BICSI
(42)
BIFMA
(29)
BOMA
(20)
BSI
(102918)
CRSI
(18)
IAHSS
(1)
IAPMO
(397)
ICC
(328)
IEC
(10763)
IEEE
(7368)
IES
(266)
ISEA
(21)
ISO
(24376)
Joint Commission
(206)
Joint Commission Int.
(1)
NEMA
(1010)
NETA
(7)
NFPA
(1737)
TCNA
(26)
TMS
(19)
USGBC
(28)
WILEY
(4)
 
AR
(6)
AZ
(7)
CA
(128)
CO
(1)
CT
(4)
FL
(58)
ID
(5)
IL
(7)
IN
(1)
KY
(1)
LA
(2)
MI
(28)
MN
(27)
NC
(36)
NJ
(13)
NY
(71)
OH
(24)
OR
(27)
PA
(7)
RI
(8)
SC
(20)
SD
(1)
TX
(3)
VA
(50)
VT
(7)
WA
(34)
CONTENT TYPE
 Act
 Admin Code
 Announcements
 Bill
 Book
 CADD File
 CAN
 CEU
 Charter
 Checklist
 City Code
 Code
 Commentary
 Comprehensive Plan
 Conference Paper
 County Code
 Course
 DHS Documents
 Document
 Errata
 Executive Regulation
 Federal Guideline
 Firm Content
 Guideline
 Handbook
 Interpretation
 Journal
 Land Use and Development
 Law
 Legislative Rule
 Local Amendment
 Local Code
 Local Document
 Local Regulation
 Local Standards
 Manual
 Model Code
 Model Standard
 Notice
 Ordinance
 Other
 Paperback
 PASS
 Periodicals
 PIN
 Plan
 Policy
 Product
 Product - Data Sheet
 Program
 Provisions
 Requirements
 Revisions
 Rules & Regulations
 Standards
 State Amendment
 State Code
 State Manual
 State Plan
 State Standards
 Statute
 Study Guide
 Supplement
 Sustainability
 Technical Bulletin
 All
  • ASTM
    E3527-26 Standard Guide for Preservation of Microorganisms
    Edition: 2026
    $144.00
    Unlimited Users per year

Description of ASTM-E3527 2026

ASTM E3527-26

Active Standard: Standard Guide for Preservation of Microorganisms




ASTM E3527

Scope

1.1 There is an ongoing need to maintain, preserve, and standardize microbiological cultures for research. This guide is focused on three parts of microbe preservation:

1.1.1 Process Description—This includes detailed microbe characterization data and documentation of microbial passaging, measurable specifications (including titers and solution concentrations), and storage conditions (for example, wet or dry storage and temperatures). Characterization data maintained from the origin of microbial isolation through preservation and disposal is a best practice. Although maintaining the purity of a microbiological culture is ideal in most circumstances, many original environmental isolates or clinical samples may be mixtures. Also, certain organisms, including mutualistic biofilm organisms and lichens or obligate pathogenic organisms (for example, viruses), may be best preserved in conjunction with symbiotic organisms or host cells.

1.1.2 Microbe Preservation Tools—Descriptions, monitoring, and maintenance of equipment used in preservation may be documented in laboratory plans or standard operating procedures (SOPs), or preferably both as a best practice. Also, detailed documentation of any personal protective equipment (PPE) requirements and the various mechanisms for administrative control (for example, controlled access to organisms) and engineering control (maintenance of sterile culture and verifications of identities of preserved microbes) may be maintained.

1.2 In this guide, methods for characterization, storage, maintenance, and stabilization of a broad taxonomy of microorganisms are reviewed, including vegetative bacteria, bacterial spores, mycobacteria, fungi, and viruses.

1.3 This guide is intended to assist in the development of standardized methods for preservation of microorganisms by indicating actions to be considered at each stage of accession and preservation.

1.4 This guide does not purport to detail the specific workflow necessary to developing a standardized method for preservation of a specific microorganism. Literature review and empirical evidence may be considered in the process of developing a final method specific to the microorganism of interest.

1.5 Units—The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


Keywords

ambient; bacteria; cryogenic; freeze-drying; freezing; fungi; microorganisms; preservation; storage; virus;


ICS Code

ICS Number Code n/a


DOI: 10.1520/E3527-26



This book also exists in the following packages...

Year Publisher Title Annual Price
VAR
ASTM
 [+] $1,419.96 Buy
VAR
ASTM
 [+] $7,192.78 Buy

Subscription Information

MADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.

 

Some features of MADCAD.com ASTM Standards Subscriptions are:

- Online access: With MADCAD.com’ s web based subscription service no downloads or installations are required. Access ASTM Standards from any browser on your computer, tablet or smart phone.

- Immediate Access: As soon as the transaction is completed, your ASTM Standards Subscription will be ready for access.

 

For any further information on MADCAD.com ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.

 

About ASTM

ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide.
GROUPS
X